Simple • Affordable • Next Generation Sequencing
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Simplify Sequencing with ClickSeq™ Technology


Affordable, Efficient, and Cutting-Edge RNAseq and DNAseq Solutions 

ClickSeq™ is a unique method for RNAseq and DNAseq that uses ‘Click-Chemistry’ in place of commonly used biological reagents such as fragmentases and ligases. ClickSeq is a simple procedure and considerably cheaper than other commonly used alternatives. 


Sequencing Services:

At ClickSeq Technologies, we provide on-site per-sample sequencing services together with bioinformatic support. 


NGS Kits:

In partnership with baseclick GmbH (baseclick.eu), we have developed and validated the following ClickSeq-based kits for NGS library preparation:

ClickSeq: for random primed RNAseq or DNAseq

Poly(A)-ClickSeq: for 3' end focused transcriptomics

X-ClickSeq: for use with user-provided primers in the Reverse Transcription step, allowing for customized, targeted and tiled assays.

Advantages of ClickSeq™:


Request our services or contact us: info@clickseq.com


ClickSeq

RNAseq without the fragmentation or ligation.

ClickSeq Schematic

ClickSeq: Fragmentation-Free Next-Generation Sequencing via Click Ligation of Adaptors to Stochastically Terminated 3′-Azido cDNAs:

Routh et al. JMB 2015

ClickSeq was originally published in 2015 in the Journal of Molecular Biology as a method to make RNAseq libraries that do not contain artifactual chimeric reads to allow the detailed analysis of rare recombination events in RNA viruses. 

Poly(A)-ClickSeq

3' end RNAseq for simplified gene expression and alternative polyadenylation analysis

Read Coverage over Human Akt1 mRNA: Poly(A)-ClickSeq (PAC-Seq) vs RNAseq

Poly(A)-ClickSeq: click-chemistry for next-generation 3΄-end sequencing without RNA enrichment or fragmentation: 

Routh et al. NAR 2017

A method for sequencing just the 3′ ends of eukaryotic messenger RNAs by priming from poly(A) tails and using AzATP, AzGTP and AzCTP to terminate RT-PCR just upstream of the poly(A) tail in the 3’ UTR was published in Nucleic Acids Research in 2017. 

Tiled-ClickSeq

Targeted sequencing: Each tiled amplicon is derived from only one template-specific primer 

Tiled-ClickSeq Schematic

Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants:

Jaworski et al. eLife 2021

Tiled-ClickSeq leverages the ClickSeq approach to perform complete genome or gene sequencing. Using multiple tiled primers, overlapping amplicons spanning the target are generated. Tiled-ClickSeq has been optimized for the whole genome sequencing of RNA viruses including SARS-CoV-2. 

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