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Simplify Sequencing with ClickSeq™ Technology
Simplify Sequencing with ClickSeq™ Technology
Affordable, Efficient, and Cutting-Edge RNAseq and DNAseq Solutions
Affordable, Efficient, and Cutting-Edge RNAseq and DNAseq Solutions
ClickSeq™ is a unique method for RNAseq and DNAseq that uses ‘Click-Chemistry’ in place of commonly used biological reagents such as fragmentases and ligases. ClickSeq™ is a simple procedure and considerably cheaper than other commonly used alternatives.
ClickSeq™ is a unique method for RNAseq and DNAseq that uses ‘Click-Chemistry’ in place of commonly used biological reagents such as fragmentases and ligases. ClickSeq™ is a simple procedure and considerably cheaper than other commonly used alternatives.
Sequencing Services:
Sequencing Services:
At ClickSeq Technologies, we provide on-site per-sample sequencing services together with bioinformatic support.
At ClickSeq Technologies, we provide on-site per-sample sequencing services together with bioinformatic support.
NGS Kits:
NGS Kits:
In partnership with baseclick GmbH (baseclick.eu), we have developed and validated the following ClickSeq-based kits for NGS library preparation:
In partnership with baseclick GmbH (baseclick.eu), we have developed and validated the following ClickSeq-based kits for NGS library preparation:
ClickSeq: for random primed RNAseq or DNAseq
ClickSeq: for random primed RNAseq or DNAseq
Poly(A)-ClickSeq: for 3' end focused transcriptomics
Poly(A)-ClickSeq: for 3' end focused transcriptomics
X-ClickSeq: for use with user-provided primers in the Reverse Transcription step, allowing for customized, targeted and tiled assays.
X-ClickSeq: for use with user-provided primers in the Reverse Transcription step, allowing for customized, targeted and tiled assays.
Advantages of ClickSeq™:
Advantages of ClickSeq™:
No fragmentation required;
No enzymatic ligation;
Ultra-low artifactual chimera formation¹;
Specialized Transcriptomic applications;
Integrated analysis pipelines, from RNA to results;
Affordable library synthesis
RNA or DNA
Suitable for fragmented/degraded samples
ClickSeq™
RNAseq without the fragmentation or ligation.
ClickSeq Schematic
ClickSeq: Fragmentation-Free Next-Generation Sequencing via Click Ligation of Adaptors to Stochastically Terminated 3′-Azido cDNAs:
ClickSeq: Fragmentation-Free Next-Generation Sequencing via Click Ligation of Adaptors to Stochastically Terminated 3′-Azido cDNAs:
ClickSeq was originally published in 2015 in the Journal of Molecular Biology as a method to make RNAseq libraries that do not contain artifactual chimeric reads to allow the detailed analysis of rare recombination events in RNA viruses.
Poly(A)-ClickSeq
3' end RNAseq for simplified gene expression and alternative polyadenylation analysis
Read Coverage over Human Akt1 mRNA: Poly(A)-ClickSeq (PAC-Seq) vs RNAseq
Poly(A)-ClickSeq: click-chemistry for next-generation 3΄-end sequencing without RNA enrichment or fragmentation:
Poly(A)-ClickSeq: click-chemistry for next-generation 3΄-end sequencing without RNA enrichment or fragmentation:
A method for sequencing just the 3′ ends of eukaryotic messenger RNAs by priming from poly(A) tails and using AzATP, AzGTP and AzCTP to terminate RT-PCR just upstream of the poly(A) tail in the 3’ UTR was published in Nucleic Acids Research in 2017.
Tiled-ClickSeq
Targeted sequencing: Each tiled amplicon is derived from only one template-specific primer
Tiled-ClickSeq Schematic
Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants:
Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants:
Tiled-ClickSeq leverages the ClickSeq™ approach to perform complete genome or gene sequencing. Using multiple tiled primers, overlapping amplicons spanning the target are generated. Tiled-ClickSeq has been optimized for the whole genome sequencing of RNA viruses including SARS-CoV-2.
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