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Resources
Resources
Explore our extensive collection of resources, including publications, news releases, public datasets, and whitepapers. This repository serves as a valuable hub for accessing the latest research findings, stay updated on our company news, delve into publicly available datasets for your studies, and access in-depth technical insights through our whitepapers. Find the information you need conveniently all in one place.
Key Publications
Key Publications
ClickSeq Schematic
ClickSeq: Fragmentation-Free Next-Generation Sequencing via Click Ligation of Adaptors to Stochastically Terminated 3′-Azido cDNAs:
ClickSeq: Fragmentation-Free Next-Generation Sequencing via Click Ligation of Adaptors to Stochastically Terminated 3′-Azido cDNAs:
ClickSeq was originally published in 2015 in the Journal of Molecular Biology as a method to make RNAseq libraries that do not contain artifactual chimeric reads to allow the detailed analysis of rare recombination events in RNA viruses.
Read Coverage over Human Akt1 mRNA: Poly(A)-ClickSeq (PAC-Seq) vs RNAseq
Poly(A)-ClickSeq: click-chemistry for next-generation 3΄-end sequencing without RNA enrichment or fragmentation:
Poly(A)-ClickSeq: click-chemistry for next-generation 3΄-end sequencing without RNA enrichment or fragmentation:
A method for sequencing just the 3′ ends of eukaryotic messenger RNAs by priming from poly(A) tails and using AzATP, AzGTP and AzCTP to terminate RT-PCR just upstream of the poly(A) tail in the 3’ UTR was published in Nucleic Acids Research in 2017.
ClickSeq Options and Schemata
ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras:
ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras:
Since the original inception of ClickSeq, we have made a number of improvements and refinements to the protocol and provide a step-by-step guide for making ClickSeq libraries published in Methods in Molecular Biology.
Tiled-ClickSeq Schematic
Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants:
Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants:
Tiled-ClickSeq leverages the ClickSeq approach to perform complete genome or gene sequencing. Using multiple tiled primers, overlapping amplicons spanning the target are generated. Tiled-ClickSeq has been optimized for the whole genome sequencing of RNA viruses including SARS-CoV-2.
Public Seminars and Talks
Public Seminars and Talks
Discover ClickSeq: a powerful, fragmentation-free, enzyme-free NGS library preparation technology based on click chemistry.
In this expert webinar, scientists from baseclick GmbH, ClickSeq Technologies, and the University of Rochester introduce how ClickSeq overcomes key challenges in next-generation sequencing, including ligation artefacts, RNA recombination, alternative polyadenylation, and splicing detection.
Characterizing the evolution of defective interfering RNA viruses
RNA Recombination in SARS-CoV-2
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