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Resources
Resources
Explore our extensive collection of resources, including publications, news releases, public datasets, and whitepapers. This repository serves as a valuable hub for accessing the latest research findings, stay updated on our company news, delve into publicly available datasets for your studies, and access in-depth technical insights through our whitepapers. Find the information you need conveniently all in one place.
Key Publications
Key Publications
ClickSeq Schematic
ClickSeq: Fragmentation-Free Next-Generation Sequencing via Click Ligation of Adaptors to Stochastically Terminated 3′-Azido cDNAs:
ClickSeq: Fragmentation-Free Next-Generation Sequencing via Click Ligation of Adaptors to Stochastically Terminated 3′-Azido cDNAs:
ClickSeq was originally published in 2015 in the Journal of Molecular Biology as a method to make RNAseq libraries that do not contain artifactual chimeric reads to allow the detailed analysis of rare recombination events in RNA viruses.
Read Coverage over Human Akt1 mRNA: Poly(A)-ClickSeq (PAC-Seq) vs RNAseq
Poly(A)-ClickSeq: click-chemistry for next-generation 3΄-end sequencing without RNA enrichment or fragmentation:
Poly(A)-ClickSeq: click-chemistry for next-generation 3΄-end sequencing without RNA enrichment or fragmentation:
A method for sequencing just the 3′ ends of eukaryotic messenger RNAs by priming from poly(A) tails and using AzATP, AzGTP and AzCTP to terminate RT-PCR just upstream of the poly(A) tail in the 3’ UTR was published in Nucleic Acids Research in 2017.
ClickSeq Options and Schemata
ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras:
ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras:
Since the original inception of ClickSeq, we have made a number of improvements and refinements to the protocol and provide a step-by-step guide for making ClickSeq libraries published in Methods in Molecular Biology.
Tiled-ClickSeq Schematic
Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants:
Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants:
Tiled-ClickSeq leverages the ClickSeq approach to perform complete genome or gene sequencing. Using multiple tiled primers, overlapping amplicons spanning the target are generated. Tiled-ClickSeq has been optimized for the whole genome sequencing of RNA viruses including SARS-CoV-2.
Public Seminars and Talks
Public Seminars and Talks
Characterizing the evolution of defective interfering RNA viruses
ClickSeq Method to Sequence Whole Viral Genomes from Mosquito Pools
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