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ClickSeq was originally published in 2015 in the Journal of Molecular Biology as a method to make RNAseq libraries that do not contain artifactual chimeric reads to allow the detailed analysis of rare recombination events in RNA viruses.
A method for sequencing just the 3′ ends of eukaryotic messenger RNAs by priming from poly(A) tails and using AzATP, AzGTP and AzCTP to terminate RT-PCR just upstream of the poly(A) tail in the 3’ UTR was published in Nucleic Acids Research in 2017.
Since the original inception of ClickSeq, we have made a number of improvements and refinements to the protocol and provide a step-by-step guide for making ClickSeq libraries published in Methods in Molecular Biology.
Tiled-ClickSeq leverages the ClickSeq approach to perform complete genome or gene sequencing. Using multiple tiled primers, overlapping amplicons spanning the target are generated. Tiled-ClickSeq has been optimized for the whole genome sequencing of RNA viruses including SARS-CoV-2.
Public Seminars and Talks
Characterizing the evolution of defective interfering RNA viruses
ClickSeq Method to Sequence Whole Viral Genomes from Mosquito Pools