Simple • Affordable • Next Generation Sequencing
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We take pride in our rigorous development and validation processes, resulting in the creation of three highly effective ClickSeq-based NGS library preparation approaches:

ClickSeq™: RNAseq without the fragmentation or ligation.

ClickSeq Schematic

ClickSeq: Fragmentation-Free Next-Generation Sequencing via Click Ligation of Adaptors to Stochastically Terminated 3′-Azido cDNAs:

Routh et al. JMB 2015

ClickSeq was originally published in 2015 in the Journal of Molecular Biology as a method to make RNAseq libraries that do not contain artifactual chimeric reads to allow the detailed analysis of rare recombination events in RNA viruses. 

Poly(A)-ClickSeq: 3' end RNAseq for gene expression and polyadenylation analysis

Read Coverage over Human Akt1 mRNA: Poly(A)-ClickSeq (PAC-Seq) vs RNAseq

Poly(A)-ClickSeq: click-chemistry for next-generation 3΄-end sequencing without RNA enrichment or fragmentation: 

Routh et al. NAR 2017

A method for sequencing just the 3′ ends of eukaryotic messenger RNAs by priming from poly(A) tails and using AzATP, AzGTP and AzCTP to terminate RT-PCR just upstream of the poly(A) tail in the 3’ UTR was published in Nucleic Acids Research in 2017. 

Tiled-ClickSeq: Targeted sequencing with only one template-specific primer per amplicon

Tiled-ClickSeq Schematic

Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants:

Jaworski et al. eLife 2021

Tiled-ClickSeq leverages the ClickSeq approach to perform complete genome or gene sequencing. Using multiple tiled primers, overlapping amplicons spanning the target are generated. Tiled-ClickSeq has been optimized for the whole genome sequencing of RNA viruses including SARS-CoV-2.

Advantages of ClickSeq™:

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Recent Publications and Highlights

Sung, P.-y.;  Zhou, Y.;  Kao, C. C.;  Aburigh, A. A.;  Routh, A.; Roy, P., 

A multidisciplinary approach to the identification of the protein–RNA connectome in double-stranded RNA virus capsids. 

de Prisco, N.;  Ford, C.;  Elrod, N. D.;  Lee, W.;  Tang, L. C.;  Huang, K.-L.;  Lin, A.;  Ji, P.;  Jonnakuti, V. S.; Boyle, L., 

Alternative polyadenylation alters protein dosage by switching between intronic and 3′ UTR sites.

Johnson, B. A.;  Xie, X.;  Bailey, A. L.;  Kalveram, B.;  Lokugamage, K. G.;  Muruato, A.;  Zou, J.;  Zhang, X.;  Juelich, T.; Smith, J. K., 

Loss of furin cleavage site attenuates SARS-CoV-2 pathogenesis.