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Protocols

Below you will find the streamlined protocols for implementing the ClickSeq™, Poly(A)-ClickSeq™, and X-ClickSeq™ processes.

ClickSeq™ kit: fragmentation-free RNAseq

ClickSeq: Random-Primed Protocol with Single Indexing using ClickSeq KitClickSeq is a simple, fragmentation-free method for the synthesis of Next-Generation Sequencing (NGS) libraries. ClickSeq derives its name by using ‘Click-Chemistry‘ in the...

View the ClickSeq™ Kit Protocol PDF

Visit the Protocols.io ClickSeq™ Protocols Page

UserGuide_ClickSeq Library Prep_v1.3.pdf

Poly(A)-ClickSeq™ kit: 3' end RNAseq for gene expression and polyadenylation analysis

Poly(A)-ClickSeq: Poly(A)-Primed Protocol with Single Indexing using Poly(A)-ClickSeq KitPoly(A)-ClickSeq is a library preparation method used to target the 3’ ends of polyadenylated RNA, such as eukaryotic mRNAs. This technique offers an alternative to convention...

View the Poly(A)-ClickSeq™ Protocol PDF

View the Protocols.io Poly(A)-ClickSeq™ Protocol Page

UserGuide_PolyA-ClickSeq Library Prep_v1.6.pdf

X-ClickSeq™ kit: customized, targeted NGS

X-ClickSeq: Custom-Primed Protocol with Single Indexing using ClickSeq KitClickSeq is a simple, fragmentation-free method for the synthesis of Next-Generation Sequencing (NGS) libraries. ClickSeq derives its name by using ‘Click-Chemistry‘ in the...

View the X-ClickSeq™ Protocol PDF

Visit the Protocols.io X-ClickSeq™ Protocol Page

UserGuide_XClickSeq Library Prep_v1.0.pdf

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